Quantitative RT-PCR of Gene Expression

cDNAs synthesized from total RNA (Ultraspec RNA isolation system; Biotecx Laboratories) were analyzed with gene-specific primer sets (Invitrogen) and SYBR Green PCR Master mix (PerkinElmer) in an iCycler (Bio-Rad) (16 (link), 32 ). Relative gene expression levels were calculated after normalization to β-actin using the ΔΔCT method (Bio-Rad).
Real-time quantitative RT-PCR primer sets used are as follows.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Samad F., Bai H., Baik N., Haider P., Zhang Y., Rega-Kaun G., Kaun C., Prager M., Wojta J., Bui Q., Chakrabarty S., Wang J., Parmer R.J, & Miles L.A. (2021). The plasminogen receptor Plg-RKT regulates adipose function and metabolic homeostasis. Journal of thrombosis and haemostasis : JTH, 20(3), 742-754.

Publication 2021

gene-specific primer sets SYBR Green PCR Master mix iCycler ΔΔCT method

Actin Cdnas Gene Gene expression Isolation Primer Real time pcr Sybr green

Corresponding Organization : Scripps Research Institute

Other organizations : VA San Diego Healthcare System, Medical University of Vienna, Wilhelminen Hospital, Ludwig Boltzmann Institute for Cancer Research, University of California, San Diego

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Gene-specific primer sets (Invitrogen)

dependent variables

Relative gene expression levels

control variables

β-actin (used for normalization)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!