Transcriptome analysis of two invasive snails

P. canaliculata (20–25 mm shell length; 25.23 ± 0.34 g; 10 individuals) and C.cahayensis (20.4–23.2 mm shell length; 22.43 ± 0.46 g; 10 individuals) were collected without the use of chemicals and grown in the Aquatic Invasive Risk Assessment Center, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China. Tissues samples from the foot, muscle, liver, and kidney were rinsed separately with water pretreated by diethyl pyrocarbonate to cleanse the samples and inactivate RNases [32 (link)]. Total RNA of each sample was extracted using a Trizol Kit (Promega) according to the manufacturer’s instructions. RNA quality was assessed using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and RNase-free agarose gel electrophoresis, with the total RNA concentration measured using a 2100 Bioanalyzer. Equal amounts of RNA from each sampled tissue were combined for subsequent experiments and RNA purity was assessed at absorbance ratios of OD_260/280 and OD_260/230. RNA integrity was confirmed by 1% agarose gel electrophoresis.