Whole blood was collected 24 h postburn injury, maintained at room temperature for 30 min, and centrifuged at 4 °C to gain blood serum. Immediately after serum collection, an antioxidant solution (0.2% triphenylphosphine/0.2% butylated hydroxytoluene/0.1% ethylenediaminetetraacetic acid per 100 µL of serum) was added at 2% of volume and frozen at −80 °C until analysis. For analysis of lipid profiles, nine isotope-labeled oxylipins were added to the samples as internal controls at 3× volume in methanol and extracted using solid phase extraction followed by the liquid chromatography-electrospray ionization/multistage mass spectrometry UPLC/MS/MS system (Waters Acquity UPLC) and the AB Sciex 6500+ QTrap system as previously described (12 (link)).