Protocol detail

Wuling San and Allopurinol for Hyperuricemia

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The Wuling San (capsule) and allopurinol were purchased from Jiangxi Pinxin Pharmaceutical Co., Ltd. (Xinyu, China) and Shanghai Xinyi Wanxiang Pharmaceutical Co., Ltd. (Shanghai, China), respectively. Potassium oxonate (PO) and sodium carboxymethyl cellulose (CMC-Na) were purchased from Yuanye Biological Co., Ltd. (Shanghai, China). Yeast extract was bought from OXOID Ltd. (Basingstoke, UK). UA content detection kit was purchased from BioSino Bio-Technology & Science Inc. (Beijing, China). Catalase (CAT) assay kit, total antioxidant capacity (T-AOC) assay kit, malondialdehyde (MDA) assay kit, superoxide dismutase (SOD) assay kit, creatinine (Cr) assay kit, and blood urea nitrogen (BUN) assay kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The kits for the detection of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were bought from Neobioscience Technology Co., Ltd. (Shenzhen, China).

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Huang J., Lin Z., Wang Y., Ding X, & Zhang B. (2023). Wuling San Based on Network Pharmacology and in vivo Evidence Against Hyperuricemia via Improving Oxidative Stress and Inhibiting Inflammation. Drug Design, Development and Therapy, 17, 675-690.

Publication 2023

Yeast extract Catalase (CAT) assay kit total antioxidant capacity (T-AOC) assay kit malondialdehyde (MDA) assay kit superoxide dismutase (SOD) assay kit creatinine (Cr) assay kit blood urea nitrogen (BUN) assay kit (TNF-α)

Allopurinol Antioxidant Assay Biological Blood urea nitrogen Capsule Catalase Creatinine Interleukin 6 Malondialdehyde Pharmaceutical Potassium oxonate Sodium carboxymethyl cellulose Superoxide dismutase Tumor necrosis factor α Yeast

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Variable analysis

independent variables

Wuling San (capsule)
Allopurinol

dependent variables

Uric acid (UA) content
Catalase (CAT) activity
Total antioxidant capacity (T-AOC)
Malondialdehyde (MDA) content
Superoxide dismutase (SOD) activity
Creatinine (Cr) level
Blood urea nitrogen (BUN) level
Interleukin-6 (IL-6) level
Tumor necrosis factor-α (TNF-α) level

control variables

Potassium oxonate (PO)
Sodium carboxymethyl cellulose (CMC-Na)
Yeast extract

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