Antibody Detection Techniques for Proteins

Mouse anti-beta-Actin (Clone AC-15; Sigma-Aldrich), goat anti-TRPP2 (G-20; Santa Cruz Biotechnology), mouse anti-Polycystin-1 (7E12; Santa Cruz Biotechnology), chicken anti-GFP (ab13970; Abcam), mouse anti-Flag M2 (clone M2; Sigma-Aldrich), rabbit anti-V5 epitope tag (Merck Millipore), and mouse anti-V5-Tag (Clone SV5-Pk1; Bio-Rad) antibodies were obtained commercially. Mouse anti-TRPP2^698–799 antibodies have been described previously [12 , 13 (link)]. Western blot detection was performed using an anti-mouse (Dako) horse-radish peroxidase-coupled secondary antibody. Antigens were visualized by immunofluorescence using secondary goat anti-chicken Alexa Fluor 488 (Thermo Fisher Scientific).

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Hofherr A., Busch T., Huber N., Nold A., Bohn A., Viau A., Bienaimé F., Kuehn E.W., Arnold S.J, & Köttgen M. (2016). Efficient genome editing of differentiated renal epithelial cells. Pflugers Archiv, 469(2), 303-311.

Publication 2016

goat anti-TRPP2 (G-20; rabbit anti-V5 epitope tag mouse anti-V5-Tag ( goat anti-chicken Alexa Fluor 488

Alexa fluor 488 Anti antibodies Antibodies Antibody Antigens Beta actin Chicken Clone Epitope Goat Horse radish peroxidase Immunofluorescence Mouse Polycystin 1 Rabbit Western blot

Corresponding Organization : University of Freiburg

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Variable analysis

independent variables

Mouse anti-beta-Actin (Clone AC-15; Sigma-Aldrich)
Goat anti-TRPP2 (G-20; Santa Cruz Biotechnology)
Mouse anti-Polycystin-1 (7E12; Santa Cruz Biotechnology)
Chicken anti-GFP (ab13970; Abcam)
Mouse anti-Flag M2 (clone M2; Sigma-Aldrich)
Rabbit anti-V5 epitope tag (Merck Millipore)
Mouse anti-V5-Tag (Clone SV5-Pk1; Bio-Rad)
Mouse anti-TRPP2^(698–799) antibodies

dependent variables

Western blot detection
Immunofluorescence visualization

control variables

Anti-mouse (Dako) horse-radish peroxidase-coupled secondary antibody
Goat anti-chicken Alexa Fluor 488 (Thermo Fisher Scientific) secondary antibody

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