Mouse Strain Establishment and Maintenance

All mice were maintained at the Center of Genetic Engineering for Human Diseases (CGEHD) animal facility at Tokai University School of Medicine. ICR (10 to 18 weeks) and C57BL/6N (8 to 12 weeks) mice were obtained from CLEA Japan, Inc. (Tokyo, Japan). eGFP Tg mouse line, that contains a single copy eGFP transgene at the Rosa26 locus, was previously generated in our facility6 (link). All the animal experiments were performed in accordance with institutional guidelines and were approved by The Institutional Animal Care and Use Committee at Tokai University (Permit Number: #143037).