Cell Culture and Apoptosis Assay Protocol

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from GIBCO® (Invitrogen, USA). Dimethylsulfoxide (DMSO) and ethidium bromide were purchased from Sigma-Aldrich (USA). Sodium bicarbonate, neutral red (NR), and melphalan in powder form were purchased from Sigma-Aldrich (Germany). Isopropyl alcohol (biotechnology grade) was purchased from Bio Basic Inc. (USA). Methanol (analytical grade) was purchased from BDH (England). A FlexiGene DNA Kit was obtained from Qiagen (Germany). Agarose (molecular grade) was purchased from Bio-Rad (USA), and an 100 bp + 1.5 Kb DNA ladder with stain was purchased from SibEnzyme (Russia). All other reagents used in this study were purchased from Sigma Chemicals Co. (USA). An Annexin V-FITC Apoptosis Detection Kit was purchased from Bender MedSystems GmbH (Austria).