NLRP3 Inflammasome Activation Inhibition

DMSO, LPS (0111:B4), and ATP were purchased from Sigma (St. Louis, MO). RIPA lysis buffer and Nitric Oxide Assay Kit were obtained from the Beyotime Institute of Biotechnology (Shanghai, China). Rabbit anti-NLRP3 monoclonal antibody was purchased from Cell Signaling Technology (Beverly, MA). Rabbit anti-ASC polyclonal antibody and rabbit anti-caspase-1 polyclonal antibody were from Santa Cruz Biotechnology Inc (Santa Cruz CA, USA), and mouse anti-β-actin monoclonal antibody was from Protein Tech Group (Chicago, IL). Micheliolide (MCL) (Molecular Weight: 248.3, purity >99%) was isolated from the Michelia compressa (Magnoliaceae) described previously [34 (link)]. Dulbecco's Modified Eagle's Medium (DMEM) and fetal bovine serum (FBS) (04-001-1A) were obtained from HyClone Laboratories Inc (Logan, UT, USA) and Biological Industries (BI, IL), respectively. Middle brook 7H9 media were obtained from Difco (Detroit, MI, USA), and oleic acid-albumin-dextrose-catalase (OADC) supplements were from BD Biosciences (BD, Sparks, MD, USA).

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Zhang Q., Jiang X., He W., Wei K., Sun J., Qin X., Zheng Y, & Jiang X. (2017). MCL Plays an Anti-Inflammatory Role in Mycobacterium tuberculosis-Induced Immune Response by Inhibiting NF-κB and NLRP3 Inflammasome Activation. Mediators of Inflammation, 2017, 2432904.

Publication 2017

DMSO LPS (0111:B4), ATP RIPA lysis buffer Nitric Oxide Assay Kit Rabbit anti-NLRP3 monoclonal antibody mouse anti-β-actin monoclonal antibody Dulbecco's Modified Eagle's Medium (DMEM) oleic acid-albumin-dextrose-catalase (OADC) supplements

Actin Albumin Anti antibody Assay Biological Buffer Caspase 1 Catalase Dextrose Dmso Fetal bovine serum Magnoliaceae Michelia Micheliolide Monoclonal antibody Mouse Nitric oxide Oleic acid Protein Rabbit Ripa Supplements

Corresponding Organization : Shanghai University of Traditional Chinese Medicine

Other organizations : University of British Columbia, Air Force Medical University

Top 5 similar protocols

Variable analysis

independent variables

LPS (0111:B4)
Micheliolide (MCL)

dependent variables

Nitric Oxide (measured using Nitric Oxide Assay Kit)

control variables

Dulbecco's Modified Eagle's Medium (DMEM)
Fetal bovine serum (FBS)
Middle brook 7H9 media
Oleic acid-albumin-dextrose-catalase (OADC) supplements

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