Dopamine Preparation in Artificial CSF

All chemicals were purchased from Sigma Aldrich (St. Louis, MO). Stock solutions of dopamine HCl (10 mM) were made in 0.1 N perchloric acid and diluted to the desired concentration the day of experiment in artificial cerebrospinal fluid (aCSF) described previously [19 (link)] and comprised of (in mM): 125 NaCl, 4 KCl, 1.3 CaCl₂, 1 MgCl₂, 0.66 NaH₂PO₄, 2 Na₂HPO₄, 1 glucose, pH 7.4. All solutions were made in 18 mOhm water (Barnstead E-Pure, Thermo Scientific, Waltham, MA).

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Ramsson E.S., Cholger D., Dionise A., Poirier N., Andrus A, & Curtiss R. (2015). Characterization of Fast-Scan Cyclic Voltammetric Electrodes Using Paraffin as an Effective Sealant with In Vitro and In Vivo Applications. PLoS ONE, 10(10), e0141340.

Publication 2015

Barnstead E-Pure

Cerebrospinal fluid Dopamine hcl Glucose Mgcl2 Nacl Perchloric acid

Corresponding Organization : Grand Valley State University

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Variable analysis

independent variables

Dopamine HCl concentration

dependent variables

Not explicitly mentioned

control variables

Composition of artificial cerebrospinal fluid (aCSF)
PH of solutions (7.4)
Water purity (18 mOhm)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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