Assessing Coral Oocyte Viability

An ATP Assay was performed for determining the viability of the coral oocytes. The productivity of the mitochondria, as indicated by the number of ATPs, indicated the degree of harm for the oocytes in a treatment. Coral oocytes were placed in a test tube, and seawater was drawn using a pipette. Oocyte viability was assessed through a luminescence assay (ApoSENSOR Cell Viability Assay Kit, BioVision, Cambridge BioScience, UK). In brief, a nucleotide-releasing buffer (100 μL) was added to the test tube, and after 3–5 min, an ATP-monitoring enzyme (5 μL) was added. After approximately 30 s to 1 min, the test tube was placed into a luminometer (Lumat LB 9507, Berthold Technologies, Germany), and readings were taken27 (link).