Isolation of polyA+ mRNA (High Pure RNA Isolation Kit; Roche Life Science, 11828665001), RT to generate cDNA (Transcriptor RTase; Roche Life Science, 3531317001) and qPCR were done according to manufacturer’s protocols. The abundance of various mRNAs was quantified by RT–qPCR relative to the stable housekeeping transcript, TBP. Gene-specific primers have been described72 (link) and are listed in Supplementary Table 1.
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