Immunoblotting of Signaling Proteins

WB was performed as previously described^{27 (link)}. The following primary antibodies were used: TC2N rabbit polyclonal antibody (1:500; Abcam), p85 mouse monoclonal antibody (1:1000; Santa Cruz), p-p85 Tyr508 goat polyclonal antibody (1:1000; Santa Cruz), p-p55γ Tyr199 rabbit polyclonal antibody (1:1000; Bioss), p55γ mouse monoclonal antibody (1:1000; Santa Cruz), AKT mouse monoclonal antibody (1:1000; Santa Cruz), p-AKT Ser473 mouse monoclonal antibody (1:1000; Santa Cruz) GSK3B mouse monoclonal antibody (1:1000; Santa Cruz), p-GSK3B mouse monoclonal antibody (1:1000; Santa Cruz), p-MYC mouse monoclonal antibody (1:1000; Santa Cruz), p-BAD mouse monoclonal antibody (1:1000; Santa Cruz), Caspase-3 mouse monoclonal antibody (1:1000; Santa Cruz), cleaved-Caspase-3 rabbit monoclonal antibody (1:1000; Cell Signaling Technology), ALK mouse monoclonal antibody (1:1000; Santa Cruz), EBP1 mouse monoclonal antibody (1:1000; Santa Cruz) and ACTIN monoclonal antibody (1:2000; Sigma). ACTIN served as a loading control.

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Hao X.L., Gao L.Y., Deng X.J., Han F., Chen H.Q., Jiang X., Liu W.B., Wang D.D., Chen J.P., Cui Z.H., Ao L., Cao J, & Liu J.Y. (2019). Identification of TC2N as a novel promising suppressor of PI3K-AKT signaling in breast cancer. Cell Death & Disease, 10(6), 424.

Publication 2019

TC2N rabbit polyclonal antibody AKT mouse monoclonal antibody cleaved-Caspase-3 rabbit monoclonal antibody ACTIN monoclonal antibody

Actin Antibodies Antibody Caspase 3 Goat Monoclonal antibody Mouse Rabbit

Corresponding Organization :

Other organizations : Army Medical University, Xinxiang Medical University

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Protocol cited in 1 other protocol

Variable analysis

independent variables

TC2N rabbit polyclonal antibody (1:500; Abcam)
P85 mouse monoclonal antibody (1:1000; Santa Cruz)
P-p85 Tyr508 goat polyclonal antibody (1:1000; Santa Cruz)
P-p55γ Tyr199 rabbit polyclonal antibody (1:1000; Bioss)
P55γ mouse monoclonal antibody (1:1000; Santa Cruz)
AKT mouse monoclonal antibody (1:1000; Santa Cruz)
P-AKT Ser473 mouse monoclonal antibody (1:1000; Santa Cruz)
GSK3B mouse monoclonal antibody (1:1000; Santa Cruz)
P-GSK3B mouse monoclonal antibody (1:1000; Santa Cruz)
P-MYC mouse monoclonal antibody (1:1000; Santa Cruz)
P-BAD mouse monoclonal antibody (1:1000; Santa Cruz)
Caspase-3 mouse monoclonal antibody (1:1000; Santa Cruz)
Cleaved-Caspase-3 rabbit monoclonal antibody (1:1000; Cell Signaling Technology)
ALK mouse monoclonal antibody (1:1000; Santa Cruz)
EBP1 mouse monoclonal antibody (1:1000; Santa Cruz)

dependent variables

Not explicitly mentioned

control variables

ACTIN monoclonal antibody (1:2000; Sigma)

controls

ACTIN served as a loading control

Annotations

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