Antibody and Inhibitor Usage in MELK Study

Following antibodies were used in this study: Anti-human MELK antibody (in-house, previously described [14 (link)]), anti-human DEPDC1 (in-house, previously described [36 (link)]), anti-human FOXM1, anti-human p53 (Santa Cruz Biotechnology), anti-human Ki67 (Millipore), anti-human p21, anti-human Slug, anti-human Snail (Cell Signaling Technology), anti-human E-cadherin (BD biosciences), anti-HA (Roche), anti-FLAG M2, and anti-beta-actin antibodies (Sigma). OTS167 was dissolved in DMSO (Sigma) for cell culture or in 5% glucose solution for animal study. The target sequences of oligo siRNAs were 5′-CUUACGCUGAGUACUUCGAUU-3′ for MELK and 5′-AGUUCAUUGGAACUACCAAUU-3′ for Luciferase (control). DEPDC1 oligo siRNA was purchased from Santa Cruz Biotechnology (sc-78918).

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Chung S., Kijima K., Kudo A., Fujisawa Y., Harada Y., Taira A., Takamatsu N., Miyamoto T., Matsuo Y, & Nakamura Y. (2016). Preclinical evaluation of biomarkers associated with antitumor activity of MELK inhibitor. Oncotarget, 7(14), 18171-18182.

Publication 2016

anti-human FOXM1 anti-human p53 anti-human Slug anti-human Snail anti-human E-cadherin anti-HA anti-FLAG M2 anti-beta-actin antibodies DMSO

Animal Anti antibodies Anti antibody Antibodies Beta actin Cell culture Depdc1 Dmso Glucose Human Human e cadherin Luciferase Oligo Ots167 Sirna Slug Snail

Corresponding Organization : University of Chicago

Other organizations : OncoTherapy Science (Japan)

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Variable analysis

independent variables

OTS167
MELK siRNA
Luciferase siRNA (control)

dependent variables

MELK protein expression
DEPDC1 protein expression
FOXM1 protein expression
P53 protein expression
Ki67 protein expression
P21 protein expression
Slug protein expression
Snail protein expression
E-cadherin protein expression

control variables

Anti-HA antibody
Anti-FLAG M2 antibody
Anti-beta-actin antibody

positive controls

Previously described anti-human MELK antibody [14]
Previously described anti-human DEPDC1 antibody [36]

negative controls

Luciferase siRNA

Annotations

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