Quantifying Neutrophil Activation Markers

Different markers of neutrophil activation were quantified in the plasma sample obtained before surgery, following the strategy addressed in previous studies [51 (link),52 (link),53 (link),54 (link),55 (link),56 (link),62 (link)] and following manufacturer´s instructions. These markers were: cfDNA (Quant-iT PicoGreen dsDNA kit, Life Technologies, Eugene, OR, USA) and nucleosomes (Cell Death Detection ELISA^PLUS kit, Roche, Mannheim, Germany) as markers of the neutrophil nuclear content released upon NETosis; calprotectin (Human Calprotectin ELISA kit, Hycult Biotech, Uden, The Netherlands) as marker of cytoplasmic content; and MPO (Human MPO ELISA kit, Abnova, Taoyuan, Taiwan) as marker of granules content, both released upon neutrophil activation by different mechanisms.

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Oto J., Plana E., Solmoirago M.J., Fernández-Pardo Á., Hervás D., Cana F., España F., Artoni A., Bucciarelli P., Carrabba G., Navarro S., Merati G, & Medina P. (2020). microRNAs and Markers of Neutrophil Activation as Predictors of Early Incidental Post-Surgical Pulmonary Embolism in Patients with Intracranial Tumors. Cancers, 12(6), 1536.

Publication 2020

Quant-iT PicoGreen dsDNA kit Cell Death Detection ELISAPLUS kit Human Calprotectin ELISA kit Human MPO ELISA kit

Calprotectin Cell death Cfdna Cytoplasmic Dsdna Elisa Granules Human Neutrophil Neutrophil activation Nucleosomes Picogreen Plasma Surgery

Corresponding Organization : Instituto de Investigación Sanitaria La Fe

Other organizations : Hospital Universitari i Politècnic La Fe, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

CfDNA (Quant-iT PicoGreen dsDNA kit, Life Technologies, Eugene, OR, USA)
Nucleosomes (Cell Death Detection ELISA^PLUS kit, Roche, Mannheim, Germany)
Calprotectin (Human Calprotectin ELISA kit, Hycult Biotech, Uden, The Netherlands)
MPO (Human MPO ELISA kit, Abnova, Taoyuan, Taiwan)

control variables

None explicitly mentioned

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

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