Quantifying Gene Expression in Myocardial Infarction

Frozen myocardial tissue from the infarcted, adjacent, and remote areas of the pigs was homogenized in TRIzol isolation reagent (Life Technologies, Madrid, Spain) for RNA isolation. RT-qPCR was performed using an ABI Prism 7900 sequence detection system (Life Technologies, Madrid, Spain) with TaqMan Gene Expression Assays (Life Technologies, Madrid, Spain). The fold change in gene expression from the control group was calculated using the 2^−ΔΔCt method [10 (link)].

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Forteza M.J., Trapero I., Hervas A., de Dios E., Ruiz-Sauri A., Minana G., Bonanad C., Gómez C., Oltra R., Rios-Navarro C., Ketelhuth D.F., Nunez J., Chorro F.J, & Bodi V. (2018). Apoptosis and Mobilization of Lymphocytes to Cardiac Tissue Is Associated with Myocardial Infarction in a Reperfused Porcine Model and Infarct Size in Post-PCI Patients. Oxidative Medicine and Cellular Longevity, 2018, 1975167.

Publication 2018

TRIzol isolation reagent ABI Prism 7900 TaqMan Gene Expression Assays

Assays Frozen Gene expression Isolation Myocardial tissue Pigs Prism Tissue Trizol

Corresponding Organization : Centro de Investigación en Red en Enfermedades Cardiovasculares

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Variable analysis

independent variables

Tissue location (infarcted, adjacent, remote)

dependent variables

Gene expression

control variables

Control group

controls

Positive control: Not specified
Negative control: Not specified

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