Two weeks before the tournament, saliva samples were obtained at (9:00 am and 8:00 pm) following a previously described protocol [29 (link)]. Saliva samples were frozen at -20°C and stored until analysis. Urine samples were also collected to analyze on-site specific gravity with a pre-calibrated hand-held refractometer (URC-Ne, Atago, Japan) as previously described[30 (link)]. During the match day, after saliva and urine collection, participant’s body weight (Tanita B-601, Tokyo, Japan) and height (Seca 202, Hamburg, Germany) were measured. In addition, before and after the match, neuromuscular tests were conducted.
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