Protocol detail

Fluorescence-Force Spectroscopy of Flagelliform Peptide-DNA Conjugate

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The 40 aa flagelliform peptide (GPGGA)₈ was crosslinked to maleimide-functionalized DNA. The peptide/DNA conjugate was labelled with organic fluorophores for fluorescence-force spectroscopy to determine the force per end-to-end distance of the peptide. For more detailed information see Online Methods and Supplementary Note III.

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Grashoff C., Hoffman B.D., Brenner M.D., Zhou R., Parsons M., Yang M.T., McLean M.A., Sligar S.G., Chen C.S., Ha T, & Schwartz M.A. (2010). Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature, 466(7303), 263-266.

Publication 2010

Fluorescence spectroscopy Maleimide Peptide

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Protocol cited in 21 other protocols

Variable analysis

independent variables

The 40 aa flagelliform peptide (GPGGA)₈ was crosslinked to maleimide-functionalized DNA.

dependent variables

The force per end-to-end distance of the peptide, determined by fluorescence-force spectroscopy.

control variables

Not explicitly mentioned.

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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