The downregulation of EphA2 and EphA3 was investigated as described previously.43 (link) Western blots for testing the presence of receptors were also performed as described previously.9 (link) Three independent experiments were performed for each QUAD version and the EphA2 and EphA3 receptors. Density of immunoreactive bands was measured on an AI600 RGB imager (GE). Data from 3 independent experiments were normalized to the corresponding β-actin and analyzed in Prism GraphPad.
Protocol detail
Investigating EphA2 and EphA3 Downregulation
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Actin
Blots testing western
Downregulation
Epha3
Epha3 receptors
Prism
Corresponding Organization : Atrium Health Wake Forest Baptist
Other organizations : Children's Hospital of Philadelphia, Wake Forest University, Virginia–Maryland College of Veterinary Medicine
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Variable analysis
independent variables
- QUAD version
- Density of immunoreactive bands for EphA2 receptor
- Density of immunoreactive bands for EphA3 receptor
- β-actin
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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