Immunofluorescence Staining of Sirt1 Protein

Immunofluorescence staining was performed as previously described [16 (link), 18 ]. Briefly, after the section was repaired, the slices were incubated with 5% BSA at room temperature for 30 min. The slices were incubated at 4 °C overnight with the following primary antibodies: Sirt1 antibody (1:100, bs-0921R, Bioss). Then, the slices were incubated with the appropriate fluorescence-conjugated secondary antibody (1: 500, Abcam, MA, USA) at 37° C for 1 h.

Free full text: Click here

Huo A, & Wang F. (2024). Berberine alleviates ischemia reperfusion injury induced AKI by regulation of intestinal microbiota and reducing intestinal inflammation. BMC Complementary Medicine and Therapies, 24, 66.

Publication 2024

bs-0921R fluorescence-conjugated secondary antibody

Corresponding Organization :

Other organizations : Tianjin Medical University, Tianjin Third Central Hospital

Top 5 similar protocols

Variable analysis

independent variables

Immunofluorescence staining protocol

dependent variables

Sirt1 protein expression

control variables

Incubation of sections with 5% BSA at room temperature for 30 min
Incubation of sections with primary antibody (Sirt1 antibody, 1:100) at 4 °C overnight
Incubation of sections with fluorescence-conjugated secondary antibody (1:500) at 37 °C for 1 h

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!