The pH value of the samples was recorded with a digital pH meter (HI 2211 pH-ORP Meter, HANNA Instruments, Woonsocket, RI, USA), during storage at different temperatures at different time intervals. After finishing the microbiological analysis, the ham homogenate (stomacher homogenate) was used to measure the pH of the samples.
The color of the ham samples was evaluated by taking at least five random readings from the surface of the different samples using a Minolta Chroma Meter fitted with CR-300 measuring head (Minolta, Osaka, Japan). Measurements of the instrument were standardized with respect to a white calibration plate, every time before use. The CIE (Commission Internationale de l’Eclairage), L*, a*, b*, colorimetry system was used for color determination with L* representing lightness, a* representing redness and b* representing yellowness. All of the measurements were collected from areas on the ham surface without visual excess fat, and the values were recorded for C* (chroma) calculation using the following equation: C* = (a*2 + b*2)1/2. Values regarding control and OEOF samples (without essential oil), were common with those reported in our previous paper [52 (link)], while the results relevant to the essential oil supplemented films—OEOS (which is the purpose of this paper) are reported for first time in the current paper.
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