FFPE tumor samples (n = 25 for mCRC and n = 20 for NSCLC) were microdissected (microdissector LMD2000, Leica, Germany, EU) and DNA was purified from the areas of the samples with the highest percentage of tumor cells using the QIAamp DNA FFPE Tissue kit (Qiagen, Cat No./ID: 56404, Valencia, CA, USA) as per manufacturer's instructions. These samples were then analyzed using a customized Ampliseq library and next-generation sequencing (NGS) on the Ion PGM (Life Technologies, Carlsbad, CA, USA).
Plasma was prepared from 30 mL of blood collected in K2 EDTA tubes (BD, 367525, 18 mg). All blood samples were delivered to the laboratory within 24 hours after collection. Detailed pre-analytical considerations have been previously published [13 (link)]. The haploid GE corresponds to the haploid Genome Equivalent (330 GE blood DNA for 1 ng/μL cfDNA).
Plasma was prepared from 30 mL of blood collected in K2 EDTA tubes (BD, 367525, 18 mg). All blood samples were delivered to the laboratory within 24 hours after collection. Detailed pre-analytical considerations have been previously published [13 (link)]. The haploid GE corresponds to the haploid Genome Equivalent (330 GE blood DNA for 1 ng/μL cfDNA).
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