Molecular Weight Analysis of Starch

As described by Zhang et al. (14 (link)), the molecular structure was analyzed by determining the molecular weight distribution. Completely dissolved solution (starch samples/dimethyl sulfoxide was 2 mg/mL, 90°C, 24 h) was evaluated with an absolute molecular weight analysis system including multi-angle laser light-scattering detector (Wyatt Technology Corporation, Santa Barbara, CA, USA), refractive index detector (Wyatt Technologies), and high-performance size-exclusion chromatography (Wyatt Technology Corporation). The guard column, Shodex OHpak SB-804 HQ and Shodex OHpak SB-806 HQ (Showa Denko K.K., Tokyo, Japan) Phenogel columns were used. The column temperature was 60°C, and flow rate of the dimethyl sulfoxide mobile phase was 0.3 mL/min. The sample injection consisted of 100 μL. Data obtained using this system were analyzed with Astra software (version 5.3.4, Wyatt Technology).

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Li B., Zhang Y., Luo W., Liu J, & Huang C. (2022). Effect of new type extrusion modification technology on supramolecular structure and in vitro glycemic release characteristics of starches with various estimated glycemic indices. Frontiers in Nutrition, 9, 985929.

Publication 2022

multi-angle laser light-scattering detector refractive index detector OHpak SB-804 HQ OHpak SB-806 HQ Astra software

Dimethyl sulfoxide Light Molecular structure Size exclusion chromatography Starch

Corresponding Organization : Guangxi University

Other organizations : Tropical Crops Genetic Resources Institute, Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences

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Variable analysis

independent variables

Starch samples/dimethyl sulfoxide concentration (2 mg/mL)
Dissolution temperature (90°C)
Dissolution time (24 h)

dependent variables

Molecular weight distribution

control variables

Guard column (Shodex OHpak SB-804 HQ and Shodex OHpak SB-806 HQ)
Column temperature (60°C)
Flow rate of the dimethyl sulfoxide mobile phase (0.3 mL/min)
Sample injection volume (100 μL)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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