Protocol detail

Identification of Bacterial Isolates

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Clinical specimens were grown aseptically on Columbia blood agar base supplemented with 5% of defibrinated sheep blood (Oxoid), chocolate blood agar (GC II agar with IsoVitaleX, BD) and MacConkey agar (Biolife). All plates were incubated at 35–37 °C in aerobic conditions with 5–10% CO₂ for 24–48 h. Identification of isolates was carried out by matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass espectrometry (Biotyper System, Bruker Daltonics, Bremen, Germany) as previously described [24 (link)].

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Petrocchi-Rilo M., Gutiérrez-Martín C.B., Pérez-Fernández E., Vilaró A., Fraile L, & Martínez-Martínez S. (2020). Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics, 9(9), 614.

Publication 2020

Columbia blood agar base defibrinated sheep blood IsoVitaleX MacConkey agar Biotyper System

Aerobic Agar Blood Chocolate Matrix assisted laser desorption ionization Sheep

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Variable analysis

independent variables

Growth media (Columbia blood agar base, chocolate blood agar, MacConkey agar)

dependent variables

Identification of isolates by MALDI-TOF mass spectrometry

control variables

Aseptic handling of clinical specimens
Incubation temperature (35-37°C)
Incubation atmosphere (aerobic with 5-10% CO2)
Incubation duration (24-48 hours)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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