Chromatin immunoprecipitation sequencing was performed with 1 × 107 cells as previously described (Liang et al. 2015 (link)). The sheared chromatin was immunoprecipitated with 10 µg individual antibodies and 15 µL preblocked Protein A/G beads (Smart-Lifesciences). Library was prepared using the NEBNext ultra II DNA library prep kit for Illumina. For ChIP-Rx experiments, sonicated human chromatin was spiked-in with 10%–30% mouse chromatin to quantitatively normalize across experiments. Experimental details and sequencing data analysis are included in Supplemental Methods .
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Chromatin Immunoprecipitation Sequencing Protocol
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Other organizations : Wuhan University
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Variable analysis
independent variables
- Chromatin immunoprecipitation
- Antibodies used for immunoprecipitation
- Sheared chromatin
- Library preparation for Illumina sequencing
- Cell count (1 × 10^7 cells)
- Preblocked Protein A/G beads (15 µL)
- 10%-30% mouse chromatin spiked-in for ChIP-Rx experiments
- Not explicitly mentioned
- Not explicitly mentioned
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