pIFNβ-Luc reporter plasmid and RIG-I expression plasmid were obtained from Dr Takashi Fujita (Kyoto University, Kyoto, Japan).15 (link) Expression vectors for TBK1, IRF3 and TRAF3 were generous gifts from Dr Genhong Cheng (University of California, Los Angeles, CA, USA).16 (link),17 (link) pISRE-Luc reporter construct was purchased from Clontech (Mountain View, CA, USA). MDA5, IKKε, MAVS and TANK expression vectors have been described.14 (link)Monoclonal anti-FLAG (clone M2) and anti-α-tubulin (clone DM1A) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-myc was bought from Santa Cruz (Dallas, TX, USA). Anti-V5 was from Life Technologies (Grand Island, NY, USA). Anti-GM130 was from BD Transduction (Lexington, KY, USA). Anti-calnexin was from Affinity Bioreagent (Golden, CO, USA).
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Plasmid and Antibody Sources for RIG-I Signaling
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Other organizations : University of Hong Kong
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Variable analysis
independent variables
- PIFNβ-Luc reporter plasmid
- RIG-I expression plasmid
- TBK1 expression vector
- IRF3 expression vector
- TRAF3 expression vector
- MDA5 expression vector
- IKKε expression vector
- MAVS expression vector
- TANK expression vector
- Luciferase activity (from pIFNβ-Luc reporter plasmid)
- PISRE-Luc reporter construct
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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