Digging and marble burying (DMB) were measured as described by Deacon (Deacon, 2006b (link)). Testing was conducted under dim lighting (20 lux). The mouse was placed in a new, clean cage with a bedding thickness of 5 cm and no lid, and allowed to freely dig for 3 min. The number of digging bouts and total digging duration were recorded (phase 1). The mouse was then removed from the cage, the bedding flattened and 12 marbles arranged in a 4 × 3 array on top of the bedding. The mouse was reintroduced into the cage and allowed to bury the marbles for 30 min with a lid covering the cage. The number of marbles that were buried (two-thirds or more) was counted at the end of the test (phase 2).
Protocol detail
Murine Digging and Marble Burying Assay
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Deacon
Dig 3
Marbles
Mouse
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Variable analysis
independent variables
- Lighting condition (dim lighting at 20 lux)
- Number of digging bouts (phase 1)
- Total digging duration (phase 1)
- Number of marbles buried (two-thirds or more) (phase 2)
- Bedding thickness (5 cm)
- Cage without a lid
- Arrangement of 12 marbles in a 4 × 3 array on top of the bedding (phase 2)
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