A SD-OCT device (Bioptigen Inc. Durham. NC, USA) was used to record the retinal thickness 20 (link). Images were captured with the rectangular scan at 1000 A-scans per B-scan, and 100 B-scans per frame. Total retinal thickness (TRT) was measured perpendicular to the surface of retinal pigment epithelial (RPE) layer and retinal nerve fiber layer (RNFL) at 12, 3, 6, and 9 o’clock positions, 500 μm away from the center of optic nerve head (ONH) using the built-in software (InVivoVU, Bioptigen), and then averaged. The examiners were blinded to the treatment information.
Protocol detail
Retinal Thickness Measurement using SD-OCT
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Device
Frame
Nerve fiber
Optic nerve head
Retinal
Retinal pigment
Scans
Corresponding Organization : Virginia Commonwealth University
Other organizations : Xiamen University, Tianjin Medical University, Tianjin Medical University Eye Hospital, Sun Yat-sen University
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Variable analysis
independent variables
- None explicitly mentioned
- Total retinal thickness (TRT)
- Location of measurement: 12, 3, 6, and 9 o'clock positions, 500 μm away from the center of optic nerve head (ONH)
- Measurement method: Perpendicular to the surface of retinal pigment epithelial (RPE) layer and retinal nerve fiber layer (RNFL)
- Imaging device: Bioptigen Inc. SD-OCT device
- Scan parameters: Rectangular scan at 1000 A-scans per B-scan, and 100 B-scans per frame
- Analysis software: InVivoVU by Bioptigen
- Examiners were blinded to the treatment information
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