Characterization of C9orf72 and STING Mice

All mice were housed in pathogen-free facilities under 12-h light dark cycles with access to food and water ad libitum. Temperature was set 74°F +/− 2° with humidity 30–70%. C9orf72^−/− mice were as previously published^{7 (link)} and wildtype mice were purchased from Jackson Laboratories. C9orf72^fl/fl mice were provided by the Pasterkamp lab^{12 (link)} and were crossed with Cx3cr1^Cre (B6J.B6N(Cg)-Cx3cr1^{tm1.1(cre)Jung}/J) and Lyz2^Cre (B6.129P2-Lyz2^tm1(cre)Ifo/J) purchased from Jackson Laboratories to obtain Cx3cr1^Cre;C9orf72^fl/fl and Lyz2^Cre;C9orf72^fl/fl mice. The STING golden-ticket (STINGgt/gt) mouse was obtained from Jackson Laboratories (C57BL/6J- Tmem173gt/J). C9orf72^−/− mice were crossed with STINGgt/gt to obtain C9orf72^−/−;STING^gt/gt mice. All mice have the nuclear background of C57BL/6J. Mice were sex- and age-matched. For all experiments mice were grouped according to genotype. For EAE and B16 melanoma models genotypes were randomly separated into experimental groups. Researchers were blinded when scoring EAE, counting tumor burden in B16 melanoma model. Husbandry and behavioral tests were conducted in accordance with the protocols described by the National Institutes of Health’s Guide for the Care and Use of Animals and were approved by Cedars-Sinai Institutional Animal Care and Use Committees (IACUC #8161).