Tracking Fluorescently Labeled GDNP

Six hours after oral administration of 2 × 10¹² /dose/mouse of either PKH26 or DiR fluorescent dye (Sigma) labelled GDNP, mice were sacrificed and the small intestine, colon, MLN, spleen and liver tissues were imaged. DiR fluorescent signal was detected and measured using the Imaging Station Pearl Impulse (Li-COR Biosciences). The labeled GDNP in the gut of mice were visualized using an Odyssey CLx Imaging System (Li-COR Biosciences). PKH26 signal in frozen tissue sections was observed using a confocal laser scanning microscopy system (Nikon, Melville, NY). The method has been described previously 19 (link).

Free full text: Click here

Kumar A., Ren Y., Sundaram K., Mu J., Sriwastva M.K., Dryden G.W., Lei C., Zhang L., Yan J., Zhang X., Park J.W., Merchant M.L., Teng Y, & Zhang H.G. (2021). miR-375 prevents high-fat diet-induced insulin resistance and obesity by targeting the aryl hydrocarbon receptor and bacterial tryptophanase (tnaA) gene. Theranostics, 11(9), 4061-4077.

Publication 2021

PKH26 Imaging Station Pearl Impulse Odyssey CLx Imaging System confocal laser scanning microscopy system

Colon Confocal laser scanning microscopy Fluorescent dye Frozen sections Liver Mice Oral administration Pkh26 Small intestine Spleen Tissue

Corresponding Organization :

Other organizations : University of Louisville, Huaian First People’s Hospital, Nanjing Medical University, Louisville VA Medical Center

Top 5 similar protocols

Variable analysis

independent variables

Type of fluorescent dye (PKH26 or DiR)

dependent variables

Fluorescent signal detected and measured in the small intestine, colon, MLN, spleen, and liver tissues

control variables

Dose of fluorescent dye (2 × 10^12 /dose/mouse)
Time after oral administration (6 hours)

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!