Murine and Human B-cell Antibody Analysis

Anti-murine Abs used in these studies include: B220 (RA3-6B2), CD24 (M1/69), CD21 (7E9), CD23 (B3B4), CD4 (RM4-4) and CD44 (IM7) from Biolegend; IgMa (DS-1) and CD40 (3/23) from BD; Ly 5.1 (A20), Ly 5.2 (104), Gr1 (RB6-8C5), CD11b (M1/70), CD3 (17A2), BAFFR (eBio7H22-E16), CD93 (AA4.1), CD62L (MEL-14) and CD40L (MR1) from eBiosciences; IgM (1B4B1) and IgD (11 (link)-26 (link)) from Southern Biotech; recombinant human insulin conjugated to biotin from Fitzgerald Industries; Streptavadin (S-868) from Life Technologies. Anti-human Abs used include: CD19 (HIB19) and IgM (MHM-88) from Biolegend; CD27 (O323) from eBiosciences; CD10 (HI10a), CD24 (ML5), CD38 (HIT2), IgD (IA6-2), and BAFFR (IIC1) from BD; anti–human FITC-conjugated 9G4 antibody (18 (link)).

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Metzler G., Dai X., Thouvenel C.D., Khim S., Habib T., Buckner J.H, & Rawlings D.J. (2017). The autoimmune risk variant PTPN22 C1858T alters B cell tolerance at discrete checkpoints and differentially shapes the naïve repertoire. Journal of immunology (Baltimore, Md. : 1950), 199(7), 2249-2260.

Publication 2017

CD44 (IM7) CD19 (HIB19) and IgM (MHM-88) CD27 (O323)

Anti antibody Biotin Cd11b Cd44 Cd62l Fitc Human Insulin Murine

Corresponding Organization : University of Washington

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