Western Blot Antibody Dilutions

Primary antibodies and their working dilutions used for western blotting included: mouse monoclonal anti-FLAG epitope (clone M2, Sigma) 1:5,000; rabbit polyclonal anti-IgaA (Cano et al., 2002 (link)) 1:10,000; rabbit polyclonal anti-OmpA (gift from H. Schwarz, University of Tübingen, Germany) 1:50,000; mouse monoclonal anti-DnaK (Enzo) 1:10,000; mouse monoclonal anti-β-lactamase (gift from L.A. Fernández, CNB-CSIC, Madrid, Spain) 1:2,000; rabbit polyclonal anti-PBP3 (Castanheira et al., 2017 (link)) 1:1,000; and, rabbit polyclonal anti-PBP2 (Castanheira et al., 2020 (link)) 1:1,000. Goat polyclonal anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Bio-Rad) were used as secondary antibodies at a 1:10,000 dilution. SDS-PAGE and western blotting were performed as described (Nunez-Hernandez et al., 2014 (link)).