Serum Collection in ADPKD Monkeys

Before the ADPKD monkeys died, we collected serum by venipuncture of the saphenous vein using a 5-mm animal prick needle (Goldenrod Animal Lancet, Braintree Scientific). Then, 2 ml of blood was collected in an anticoagulant-free blood collection tube (BD vacutainer, SST II Advance). The monkeys were carefully monitored for 30 min following blood withdrawal to watch for signs of distress. An additional 0.5 ml of blood was collected into an EDTA-K2 tube. Biochemical index levels were determined by Kunming Biomed International Laboratory using a Roche Cobas C501 (Roche, Germany). Hemocytometry was performed on a Sysmex XT-2000i (Sysmex, Japan).