Isolation and Molecular Characterization of S. aureus and P. aeruginosa

The Centers for Disease Control followed inclusion guidelines in this study (Horan et al., 2008 (link)). The isolates were obtained from individuals of varying ages and genders and were not duplicated; just one sample per patient was collected. In this regard, 30 S. aureus isolates were obtained from the wound (n = 8), blood (n = 11), urine (n = 6), as well as sputum (n = 5) and further characterized and confirmed using biochemical tests like colony morphology, gram-positive, clustered-shaped cocci, catalase, mannitol, DNase, and coagulase (Murray et al., 1995 ). In addition, 20 clinical P. aeruginosa were collected from respiratory tracts retrieved from sputum (n = 6), bronchoalveolar lavage (8), and endotracheal aspirates (n = 6) patients hospitalized in the intensive care unit (ICU) wards and then confirmed on selective media via conventional phenotypical tests such as colony morphology, oxidase, catalase, motility, citrate, indole synthesis, methyl red, and voges-proskauer. Finally, molecular confirmation of S. aureus and P. aeruginosa isolates was done by the polymerase chain reaction (PCR) via previously described primers (Atshan et al., 2012 (link); Abdelraheem et al., 2020 (link)). Besides, S. aureus ATCC 25923, S. aureus ATCC 29213, and P. aeruginosa PAO1 were provided by the Pasteur Institute of Iran.

Free full text: Click here

Mirzaei R., Esmaeili Gouvarchin Ghaleh H, & Ranjbar R. (2023). Antibiofilm effect of melittin alone and in combination with conventional antibiotics toward strong biofilm of MDR-MRSA and -Pseudomonas aeruginosa. Frontiers in Microbiology, 14, 1030401.

Publication 2023

Blood Bronchoalveolar lavage Catalase Citrate Coagulase Dnase Genders Indole Mannitol Motility Oxidase P aeruginosa Patients Polymerase chain reaction Primers Respiratory tracts S aureus Sputum Synthesis Urine Wound

Corresponding Organization : Baqiyatallah University of Medical Sciences

Other organizations : Hamedan University of Medical Sciences

Top 5 similar protocols

Variable analysis

independent variables

Isolate source (wound, blood, urine, sputum, respiratory tracts)

dependent variables

Characterization and confirmation of S. aureus and P. aeruginosa isolates using biochemical tests and molecular methods

control variables

Inclusion guidelines followed by the Centers for Disease Control
Single sample per patient collected, no duplicates

controls

Positive controls: S. aureus ATCC 25923, S. aureus ATCC 29213, and P. aeruginosa PAO1 provided by the Pasteur Institute of Iran

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!