Drug crystallization
during incubation in acidic media was also evaluated by PLM using
a Nikon Eclipse E600 microscope coupled with a Nikon DS-Ri2 camera
(Melville, NY). A thin film ASD was prepared by a spin-coater KW-4A
(Chemat Technology Inc., Northridge, CA). A solution of drug, 1,2-ethanedisulfonic
acid, and polymer in organic solvents (acetone-DCM 1:1 v/v) were dropped
onto a square cover glass (22 mm × 22 mm) and kept spinning at
1000 rpm for 10s, followed by 3000 rpm for 45 s in a glove box at
the relative humidity below 20%. Similarly, thin films of ASD DLM
free base were prepared from a solution of DLM and HPMCP in MeOH-DCM
1:1 v/v. ASD films were stored under a vacuum oven overnight. Acidic
media (HCl solution pH 1.6; or phosphate buffer pH 3.0, or pH 5.0)
was added to a slide with a concave depression (Fisher Scientific,
Pittsburgh, PA). The cover glass was placed in contact with the aqueous
media. Drug crystallization on the film was visualized under PLM for
60 min using a 20× objective.
during incubation in acidic media was also evaluated by PLM using
a Nikon Eclipse E600 microscope coupled with a Nikon DS-Ri2 camera
(Melville, NY). A thin film ASD was prepared by a spin-coater KW-4A
(Chemat Technology Inc., Northridge, CA). A solution of drug, 1,2-ethanedisulfonic
acid, and polymer in organic solvents (acetone-DCM 1:1 v/v) were dropped
onto a square cover glass (22 mm × 22 mm) and kept spinning at
1000 rpm for 10s, followed by 3000 rpm for 45 s in a glove box at
the relative humidity below 20%. Similarly, thin films of ASD DLM
free base were prepared from a solution of DLM and HPMCP in MeOH-DCM
1:1 v/v. ASD films were stored under a vacuum oven overnight. Acidic
media (HCl solution pH 1.6; or phosphate buffer pH 3.0, or pH 5.0)
was added to a slide with a concave depression (Fisher Scientific,
Pittsburgh, PA). The cover glass was placed in contact with the aqueous
media. Drug crystallization on the film was visualized under PLM for
60 min using a 20× objective.
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