Genetic Manipulation of Drosophila Stocks

All stocks were maintained at 18°C on standard media, and crosses were cultured at 29°C unless otherwise indicated. Stocks used were: drm-Gal4, UAS-GFP (drm > GFP) (Green et al. 2002 (link)), smg1^32AP; btl-Gal4, UAS-Moesin::GFP (btl > MoeGFP, smg1^32AP is an allele of smg1 [courtesy of M.Metzstein]; male progeny of crosses using virgins from this stock show enhanced expression of UAS constructs due to interference with nonsense mediated decay (Metzstein and Krasnow 2006 (link)). btl > MoeGFP crosses were incubated at 25°C and high GFP progeny were assessed), UAS-trc RNAi (41591, Bloomington), UAS-luciferase RNAi (31603, Bloomington), UAS-Ccm3 RNAi (109453, VDRC), UAS-coracle RNAi (51845, TRiP), UAS-furry RNAi (60103, Bloomington), UAS-Mob2 RNAi (107327, VDRC), UAS-Mo25 RNAi (55681, Bloomington), UAS-PyK RNAi 1 and 2 (49533 & 35165, VDRC), UAS-HexA RNAi 1 and 2 (104680, VDRC and 35155, Bloomington), UAS-Pfk RNAi 1 and 2 (105666, VDRC and 36782, Bloomington), UAS-blw RNAi (28059, Bloomington), UAS-ATPsyn-beta RNAi (28056, Bloomington), and UAS-ND75 RNAi 1 and 2 (33911 and 27739, Bloomington). Refer to Supplementary Table 2 and 3 for transgenic flies used in this study.

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Hudson J., Paul S., Veraksa A., Ghabrial A., Harvey K.F, & Poon C. (2023). NDR kinase tricornered genetically interacts with Ccm3 and metabolic enzymes in Drosophila melanogaster tracheal development. G3: Genes|Genomes|Genetics, 13(3), jkad013.

Publication 2023

UAS-trc RNAi UAS-luciferase RNAi

Allele Ccm3 Flies Hexa Luciferase Male progeny Moesin Nonsense mediated decay Rnai Transgenic

Corresponding Organization :

Other organizations : Peter MacCallum Cancer Centre, University of Melbourne, University of Massachusetts Boston, Columbia University, Australian Regenerative Medicine Institute, Monash University

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Variable analysis

independent variables

Temperature: 18°C vs. 29°C
Genotypes: drm-Gal4, UAS-GFP; smg1^32AP; btl-Gal4, UAS-Moesin::GFP; UAS-trc RNAi; UAS-luciferase RNAi; UAS-Ccm3 RNAi; UAS-coracle RNAi; UAS-furry RNAi; UAS-Mob2 RNAi; UAS-Mo25 RNAi; UAS-PyK RNAi 1 and 2; UAS-HexA RNAi 1 and 2; UAS-Pfk RNAi 1 and 2; UAS-blw RNAi; UAS-ATPsyn-beta RNAi; UAS-ND75 RNAi 1 and 2

dependent variables

Expression of UAS constructs

control variables

Standard media
Stocks maintained at 18°C

positive controls

Btl > MoeGFP

negative controls

UAS-luciferase RNAi

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