Random blood glucose concentration was measured using the AlphaTrak blood glucose monitoring system (Abbott laboratories, Chicago, IL). Mice were considered diabetic once tail vein blood glucose readings of ≥ 250 mg/dL were obtained on two consecutive days. Glucose tolerance test was performed on mice every two weeks, as described previously[24 (link)]. Briefly, following overnight fasting, each mouse received 2g/ Kg body weight of glucose via intraperitoneal injection, and their blood glucose was monitored at 15, 30, 45, 60, 90, and 120 minutes post-injection.
Protocol detail
Glucose Tolerance Test in Diabetic Mice
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Blood glucose
Blood system
Body weight
Glucose
Glucose tolerance test
Intraperitoneal injection
Mouse
Tail
Vein
Corresponding Organization : Tulane University
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Variable analysis
independent variables
- Glucose administration (2g/Kg body weight via intraperitoneal injection)
- Blood glucose concentration
- Glucose tolerance
- Overnight fasting before glucose tolerance test
- Time points for blood glucose monitoring (15, 30, 45, 60, 90, and 120 minutes post-injection)
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