Metabolic Measurements in Mouse Models

Serum levels of glucose (glutest sensor; Sanwa Kagaku, Aichi, Japan), triglyceride (Triglyceride E-test; Wako Chemical, Osaka, Japan), non-esterified fatty acid (NEFA C-test; Wako Chemical, Osaka, Japan), β-hydroxybutyrate (EnzyChrom Ketone Body Assay Kit; BioAssay Systems, Milpitas, CA, USA), insulin (Mouse Insulin ELISA; Mercodia, Uppsala, Sweden) and lactate (Lactate Colorimetric Assay Kit; BioVision, Milpitas, CA, USA) were measured according to the manufacturers’ protocols [9 (link),14 (link),26 (link)]. Glycogen in hearts was measured as described previously [30 (link)].

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Umbarawan Y., Kawakami R., Syamsunarno M.R., Obinata H., Yamaguchi A., Hanaoka H., Hishiki T., Hayakawa N., Koitabashi N., Sunaga H., Matsui H., Kurabayashi M, & Iso T. (2021). Reduced Fatty Acid Use from CD36 Deficiency Deteriorates Streptozotocin-Induced Diabetic Cardiomyopathy in Mice. Metabolites, 11(12), 881.

Publication 2021

Triglyceride E-test NEFA C-test EnzyChrom Ketone Body Assay Kit Mouse Insulin ELISA Lactate Colorimetric Assay Kit

Assay Colorimetric Elisa Esterified fatty acid Glucose Glycogen Hearts Hydroxybutyrate Insulin Ketone body Lactate Mouse Nefa Serum Triglyceride

Corresponding Organization : Gunma University of Health and Welfare

Other organizations : University of Indonesia, Padjadjaran University, Keio University, Ashikaga University

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Variable analysis

dependent variables

Serum levels of glucose
Serum levels of triglyceride
Serum levels of non-esterified fatty acid
Serum levels of β-hydroxybutyrate
Serum levels of insulin
Serum levels of lactate
Glycogen in hearts

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