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Purification of CRISPR-Cas9 Protein

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Gangoso E., Southgate B., Bradley L., Rus S., Galvez-Cancino F., McGivern N., Güç E., Kapourani C.A., Byron A., Ferguson K.M., Alfazema N., Morrison G., Grant V., Blin C., Sou I., Marques-Torrejon M.A., Conde L., Parrinello S., Herrero J., Beck S., Brandner S., Brennan P.M., Bertone P., Pollard J.W., Quezada S.A., Sproul D., Frame M.C., Serrels A, & Pollard S.M. (2021). Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion. Cell, 184(9), 2454-2470.e26.
Publication 2021
BL21(DE3) cells pET28a/Cas9-Cys IPTG HisTrap HP 5 mL column HiPrep 26/10 Desalting Column
Bacterial Buffer Cas9 protein Cells Glycerol Hepes Imidazole Iptg Nacl Pellets Plasmid Tcep Tris

Corresponding Organization : University of Edinburgh

Other organizations : University College London, London Cancer, MRC Centre for Reproductive Health, The Queen's Medical Research Institute, National Hospital for Neurology and Neurosurgery, Brown University, Providence College, Centre for Inflammation Research

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