Spores of C. botulinum Beluga WT and Beluga Ei were used to inoculate CMM-TPGY in three biological replicates. Culture OD600 was measured at a wavelength of 600 nm using a Thermo Spectronic Genesys 10 UV/Vis Spectrophotometer. Heat-resistant spore and viable cell concentrations were determined as described42 (link) applying the most probable number technique57 (link). One-way ANOVA was used to compare the total viable cell and spore counts.
Nowakowska M.B., Selby K., Przykopanski A., Krüger M., Krez N., Dorner B.G., Dorner M.B., Jin R., Minton N.P., Rummel A, & Lindström M. (2022). Construction and validation of safe Clostridium botulinum Group II surrogate strain producing inactive botulinum neurotoxin type E toxoid. Scientific Reports, 12, 1790.
Anova Beluga Biological Botulinum c Cell Spore Spore counts
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Other organizations :
University of Helsinki, Medizinische Hochschule Hannover, Robert Koch Institute, University of California, Irvine, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council, University of Nottingham
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