Fecal samples submitted to the clinical laboratory were tested with the TechLab® Toxin A/B II™ EIA (Alere™, Blacksburg, VA); testing was rejected on formed fecal specimens. If available, remnant feces was frozen at −80°C. In the pre- and post-intervention period, culture for toxigenic C. difficile and Xpert C. difficile/Epi polymerase chain reaction (PCR) testing (Cepheid®, Sunnyvale, CA) was retrospectively performed on index and repeat stool specimens from patients for whom repeat testing was requested and remnant stool was available. C. difficile culture and identification were performed per previously published procedures.8 (link)
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Retrospective C. difficile Diagnostic Testing
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Corresponding Organization : Washington University in St. Louis
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Variable analysis
independent variables
- Pre- and post-intervention period
- Toxigenic C. difficile culture
- Xpert C. difficile/Epi polymerase chain reaction (PCR) testing
- Fecal sample testing with the TechLab® Toxin A/B II™ EIA (Alere™, Blacksburg, VA)
- Testing rejected on formed fecal specimens
- Remnant feces frozen at −80°C
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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