Plasmid Sources for TRIM and Signaling Proteins

TRIM1-TRIM32 and TRIM37 were obtained from Dr. Andrea Ballabio(Texaschildren’s hospital)^{53 (link)}; TRIM35, 39, 41, 43, 44, 46, 47, 52, 58, and 62 were from Dr. Walther Mothes (Yale University)^{54 (link)}; and TRIM36, 48, 49, 56 59, 60, 61, 63, and 72 were from Open Biosystems and Invitrogen. Expression plasmid for RIG-I-2CARD and TRIM25 was described previously^{27 (link)} and TRIM34 was obtained from Dr. Paul D. Bieniasz(Howard Hughes Medical Institute)^{55 (link)}., HA-NIK (Addgene plasmid 27554) was from Dr. Shao-Cong Sun(MD Anderson Cancer Center) 12. β-TrCP and NF-κB2 (p100) were received from Dr. Michele Pagano (New York University)^{56 (link)}. TRIM9 and β -TrCP mutants were generated using a PCR site-directed mutagenesis kit (Stratagene) and subcloned into pCDH vector (System Biosciences) or pEBG vector. Lentivirus-shRNAs against TRIM9 were purchased from OpenBiosystem. The plasmid pRL-TK constitutively expressing Renilla luciferase was purchased from Promega. IKKα, IKK β, p65, p50, IκBα were gifted from Dr. EbrahimZandi (University of Southern California)^{57 (link)}. Firefly luciferase reporter plasmids under the ISRE (ISG54), NF-AT, AP-1 or NF-κB promoters were purchased from Stratagene. TRAF6, TAB2, IKKε, TBK1, and ubiquitin-expressing plasmids were described previously^{29 (link)}.