Intraperitoneal Glucose Tolerance Test

An IGTT was performed on Day 55. After 12 h of fasting, 0.1 mL of blood was taken from the tail nick that rat tail was only cut once, such as other studies [31 (link)–33 (link)], and serum glucose was measured using an Accu-Check Advantage Blood Glucose Monitor (Roche Diagnostic Corporation, Indianapolis, IN, USA). Immediately after this baseline measurement, a dose of 50% glucose solution (1 g/kg body weight) was injected intraperitoneally as described previously [34 (link)]. The serum glucose level was measured at 0, 30, 60, 90, and 120 minutes after the glucose load.

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Chou C.L., Pang C.Y., Lee T.J, & Fang T.C. (2015). Beneficial Effects of Calcitriol on Hypertension, Glucose Intolerance, Impairment of Endothelium-Dependent Vascular Relaxation, and Visceral Adiposity in Fructose-Fed Hypertensive Rats. PLoS ONE, 10(3), e0119843.

Publication 2015

Accu-Check Advantage Blood Glucose Monitor

Blood Blood glucose Body weight Diagnostic Glucose Serum Tail

Corresponding Organization :

Other organizations : Tzu Chi University, Southern Illinois University School of Medicine, Taipei Medical University

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Variable analysis

independent variables

Dose of 50% glucose solution (1 g/kg body weight)

dependent variables

Serum glucose level

control variables

Fasting duration (12 h)
Blood sample volume (0.1 mL)
Sampling method (tail nick)
Glucose measurement method (Accu-Check Advantage Blood Glucose Monitor)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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