Purification and Synthesis of Biomolecules

PADs 1, 2, 3 and 4 were purified as reported.^{44 (link), 53 (link)} Rhodamine-phenylglyoxal (Rh-PG) was synthesized as reported.^{43 (link)} The plasmid pET28a-LIC, harboring the NNMT gene was purchased from Addgene (Addgene plasmid # 40734). All chemicals were purchased from Sigma.

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Nemmara V.V., Tilvawala R., Salinger A.J., Miller L., Nguyen S.H., Weerapana E, & Thompson P.R. (2018). Citrullination Inactivates Nicotinamide-N-methyltransferase. ACS chemical biology, 13(9), 2663-2672.

Publication 2018

pET28a-LIC

Gene Nnmt Phenylglyoxal Plasmid Rhodamine

Corresponding Organization : University of Massachusetts Chan Medical School

Other organizations : Boston College

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Variable analysis

independent variables

PADs 1, 2, 3 and 4 were purified
Rhodamine-phenylglyoxal (Rh-PG) was synthesized
The plasmid pET28a-LIC, harboring the NNMT gene was purchased from Addgene

dependent variables

Not explicitly mentioned

control variables

All chemicals were purchased from Sigma

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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