HPLC Analysis of Phytochemicals

HPLC-grade acetonitrile and methanol were purchased from Merck (Darmstadt,
Germany). Ultra-pure water was processed by a Milli-Q purification system
(Millipore, Molsheim, France). The chemical standards of syringin, chlorogenic
acid, isochlorogenic acid A, isochlorogenic acid B, and kaurenoic acid were
purchased from Weikeqi-Biotech Co., Ltd (Chengdu, China). All the standards have
a purity of at least 98% based on HPLC profile. Imipramine was from Yuanye
Bio-Technology Co., Ltd (Shanghai, China) and other reagents came from
Sigma-Aldrich (St Louis, MO).

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Liu C., Yan L., Qian Y., Song P., Wang T, & Wei M. (2023). The Extract of Acanthopanacis Cortex Relieves the Depression-Like Behavior and Modulates IL-17 Signaling in Chronic Mild Stress-Induced Depressive Mice. Dose-Response, 21(1), 15593258221148817.

Publication 2023

HPLC-grade acetonitrile methanol Milli-Q purification system Imipramine

Acetonitrile Hplc Imipramine Isochlorogenic acid Isochlorogenic acid b Kaurenoic acid Methanol Syringin

Corresponding Organization : Nanjing University of Chinese Medicine

Other organizations : China Pharmaceutical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

HPLC-grade acetonitrile and methanol
Ultra-pure water processed by a Milli-Q purification system
Chemical standards of syringin, chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and kaurenoic acid
Imipramine
Other reagents from Sigma-Aldrich

controls

Positive control: Chemical standards with purity of at least 98% based on HPLC profile
Negative control: Not mentioned

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