A micromanipulation system (Narishige Group, Tokyo, Japan) attached to an inverted
microscope (TE2000-S, Nikon Corporation, Tokyo, Japan) was used. The SCNT procedure was
carried out using the regimen described by Shimatsu et al. [32 (link)]. Donor cell–oocyte complexes were fused and
simultaneously activated with a single 1.7 kV/cm DC pulse for 60 µs using
a Super Electro Cell Fusion Generator (ECFG21, Nepa Gene Co., Ltd., Chiba, Japan) and then
cultured in porcine zygote medium (PZM-5, Research Institute for the Functional Peptides
Co., Ltd., Yamagata, Japan) supplemented with 2 mM 6-dimethylaminopurine for 1 h.
microscope (TE2000-S, Nikon Corporation, Tokyo, Japan) was used. The SCNT procedure was
carried out using the regimen described by Shimatsu et al. [32 (link)]. Donor cell–oocyte complexes were fused and
simultaneously activated with a single 1.7 kV/cm DC pulse for 60 µs using
a Super Electro Cell Fusion Generator (ECFG21, Nepa Gene Co., Ltd., Chiba, Japan) and then
cultured in porcine zygote medium (PZM-5, Research Institute for the Functional Peptides
Co., Ltd., Yamagata, Japan) supplemented with 2 mM 6-dimethylaminopurine for 1 h.
