Western Blot Antibody Validation Protocol

Western Blot was performed as described previously [6 (link)]. Following antibodies were used: anti-NPR3 (EPR12716, Abcam, Berlin, Germany), anti-pAKT (Ser473; #9271, Cell Signaling, Frankfurt, Germany), anti-AKT (#9272, Cell Signaling), anti-adiponectin (#GTX112777, GeneTex, Taiwan), anti-IRAK1 (SC-5287, Santa Cruz, Heidelberg, Germany), anti-IRAK1 (#4504, Cell Signaling), anti-TRAF6 (PA29622, Invitrogen, Carlsbad, USA), anti-α-tubulin (DM1A, Merck, Darmstadt, Germany), anti-β-actin (AC-15, Sigma-Aldrich), anti-GAPDH (6C5, HyTest, Turku, Finland), hFAB rhodamine anti-GAPDH, anti-tubulin, and anti-actin (#12004168, #12004166, #12004164, BioRad, Feldkirchen, Germany).

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Roos J., Dahlhaus M., Funcke J.B., Kustermann M., Strauss G., Halbgebauer D., Boldrin E., Holzmann K., Möller P., Trojanowski B.M., Baumann B., Debatin K.M., Wabitsch M, & Fischer-Posovszky P. (2020). miR-146a regulates insulin sensitivity via NPR3. Cellular and Molecular Life Sciences, 78(6), 2987-3003.

Publication 2020

anti-pAKT (Ser473; anti-AKT anti-adiponectin anti-IRAK1 anti-α-tubulin (DM1A, anti-β-actin (AC-15, hFAB rhodamine anti-GAPDH anti-tubulin anti-actin

Actin Adiponectin Antibodies Gapdh Irak1 Rhodamine Traf6 Tubulin Western blot α tubulin

Corresponding Organization :

Other organizations : Universität Ulm

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