Irradiation of Medaka Embryos

Embryos at stage 28 (30-somite stage, 64 h after fertilization) were irradiated with γ-ray emitted by ¹³⁷Cs (10 Gy, Gammacell 3000Elan, MDS Nordion, Ottawa, ON, Canada) at a dose rate of 7.5 Gy/min at room temperature in a plastic tube containing water. Medaka embryos at stage 28 correspond approximately to early fetal stage human embryos (approximately 8–15 weeks postovulation) [65 (link)]. Targeted irradiation to the right hemisphere of OT and the central part of OT of embryonic brain with a collimated microbeam (250 μm diameter) of carbon ions was conducted at the irradiation facility of TIARA (Takasaki Ion Accelerators for Advanced Radiation Application), National Institutes of Quantum Beam Science and Technology (QST) as previously reported [54 (link)]. Irradiation of whole brain of medaka embryo was conducted as reported by Nagata et al. [44 (link)].

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Yasuda T., Kamahori M., Nagata K., Watanabe-Asaka T., Suzuki M., Funayama T., Mitani H, & Oda S. (2017). Abscopal Activation of Microglia in Embryonic Fish Brain Following Targeted Irradiation with Heavy-Ion Microbeam. International Journal of Molecular Sciences, 18(7), 1428.

Publication 2017

Gammacell 3000Elan

Brain Carbon Embryo Fertilization Fetal Human Irradiation Medaka Radiation Somite Targeted irradiation

Corresponding Organization : The University of Tokyo

Other organizations : National Institutes for Quantum and Radiological Science and Technology

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Variable analysis

independent variables

Irradiation with γ-ray emitted by 137Cs at a dose of 10 Gy and a dose rate of 7.5 Gy/min
Targeted irradiation to the right hemisphere of OT and the central part of OT of embryonic brain with a collimated microbeam (250 μm diameter) of carbon ions

dependent variables

Outcomes of the irradiation on the embryos

control variables

Embryos were at stage 28 (30-somite stage, 64 h after fertilization)
Irradiation was conducted at room temperature in a plastic tube containing water
Medaka embryos at stage 28 correspond approximately to early fetal stage human embryos (approximately 8–15 weeks postovulation)

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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