Dulbecco’s Modified Essential Medium/Ham’s Nutrient Mixture (DMEM:F12), Penicillin-Streptomycin solution and 2.5g/l-Trypsin/1mmol/l-EDTA Solution were purchased from Nacalai Tesque (Tokyo, Japan). Fetal bovine serum (FBS) and non-essential amino acids (NEAA) was purchased from Gibco-BRL (Grand Island, NY). Lipopolysaccharide (LPS) from Escherichia coli O55:B5 was purchased from Merck (Darmstadt, Germany).
SH-SY5Y neuroblastoma cell line were purchased from ATCC (ATCC® CRL-2266™). The cells were initially grown in Dulbecco’s Modified Essential Medium (DMEM:F12) which contains 4.5 g/l glucose with 2mM of L-glutamine and sodium pyruvate, supplemented with 15% FBS, 1% of Penicillin-Streptomycin mixed solution and 1% NEAA at 37°C with 5% carbon dioxide (CO2). Then, the cells were induced with 10 μM of all-trans retinoic acid for 5 days in a differentiation media (DMEM:F12, supplemented with 2.5% Fetal bovine serum (FBS) and 1% of Penicillin-Streptomycin mixed solution) (Forster et al., 2016 (link); Izham et al., 2018 (link)).
SH-SY5Y neuroblastoma cell line were purchased from ATCC (ATCC® CRL-2266™). The cells were initially grown in Dulbecco’s Modified Essential Medium (DMEM:F12) which contains 4.5 g/l glucose with 2mM of L-glutamine and sodium pyruvate, supplemented with 15% FBS, 1% of Penicillin-Streptomycin mixed solution and 1% NEAA at 37°C with 5% carbon dioxide (CO2). Then, the cells were induced with 10 μM of all-trans retinoic acid for 5 days in a differentiation media (DMEM:F12, supplemented with 2.5% Fetal bovine serum (FBS) and 1% of Penicillin-Streptomycin mixed solution) (Forster et al., 2016 (link); Izham et al., 2018 (link)).
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