DNA was isolated from mouse tail and CD11c+ and CD11neg cells obtained from WT, PRnegCD11c and PRflox/wt CD11ccre/wt mice using the DNeasy Kit (Qiagen) according to the manufacturer's protocol.
PCR analysis was performed as 3-Primer-PCR in 50 μl reactions using the Mastercycler® nexus GX2 (Eppendorf). Primer sequences used were previously published (13 (link)) and ordered from TIB Molbiol. The PCR program consisted of initial 94°C for 10 min followed by 30 cycles: 94°C for 1 min, 55°C for 1 min, and 72°C for 2 min. Amplicons were visualized using Agarose gel electrophoresis. Expected band sizes were 226bp for the wildtype allele, 260 bp for the floxed allele and 381 bp for the KO allele.
PCR analysis was performed as 3-Primer-PCR in 50 μl reactions using the Mastercycler® nexus GX2 (Eppendorf). Primer sequences used were previously published (13 (link)) and ordered from TIB Molbiol. The PCR program consisted of initial 94°C for 10 min followed by 30 cycles: 94°C for 1 min, 55°C for 1 min, and 72°C for 2 min. Amplicons were visualized using Agarose gel electrophoresis. Expected band sizes were 226bp for the wildtype allele, 260 bp for the floxed allele and 381 bp for the KO allele.
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