Genome sequences were obtained by overlapping RT-PCRs based on published USUV primers [33 (link)]. A second step of RT-PCRs was done with specific primers to obtain the complete genome. cDNA synthesis was done using an AMV reverse transcription kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions, and PCR reactions were performed using Go-Taq PCR kit (Promega, Madison, WI, USA).
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